Fluorescent proteins such as mClover are invaluable tools in cell biology and molecular research. To enhance the specificity and sensitivity of detection, antibodies like the Goat anti-mClover Polyclonal IgG Antibody are essential. Below, we provide an in-depth overview of this antibody, its applications, and its benefits, supported by numerous links to educational and governmental resources.

Understanding mClover and Its Applications

mClover is a green fluorescent protein (GFP) variant, widely used in:

To maximize these applications, high-affinity antibodies like Goat anti-mClover Polyclonal IgG are essential for:

Key Features of Goat anti-mClover Polyclonal IgG Antibody

  1. High Specificity: Recognizes mClover with minimal cross-reactivity. Find details on antibody specificity.
  2. Wide Compatibility: Validated for use in multiple applications and sample types. Learn about antibody validation.
  3. Robust Performance: Delivers consistent results in demanding experimental conditions. Read about robust assay design.
  4. High Sensitivity: Detects low-abundance proteins, enabling precise analyses. Explore sensitivity in detection methods.
  5. Reproducibility: Provides consistent performance across experimental replicates. Learn about reproducibility in science.

Protocols and Usage Tips

  1. Sample Preparation: Ensure proper lysis and extraction protocols for high-quality samples. Review sample preparation guidelines.
  2. Antibody Dilution: Optimize antibody concentration for your specific application. Find optimization tips.
  3. Incubation Conditions: Use recommended incubation times and temperatures for best results. Explore incubation best practices.
  4. Validation: Test the antibody in a pilot experiment to verify its specificity and performance. Learn about validation techniques.
  5. Controls: Include positive and negative controls in your experiments to ensure reliable results. Read about experimental controls.

Applications in Research

Advantages of Goat anti-mClover Polyclonal IgG Antibody

Real-World Applications

Frequently Asked Questions (FAQs)

  1. Is the antibody suitable for FRET-based applications? Yes, it is optimized for applications involving mClover in FRET systems. Learn more about FRET compatibility.
  2. What are the storage conditions? Store at -20°C for long-term stability. Read about antibody storage guidelines.
  3. Can it be used in live-cell imaging? While primarily for fixed samples, it can complement live-cell studies. Discover live-cell imaging techniques.
  4. What sample types are compatible? The antibody works with lysates, tissue sections, and purified proteins. Find detailed compatibility guidelines.

Conclusion

The Goat anti-mClover Polyclonal IgG Antibody is an indispensable tool for researchers working with mClover-tagged proteins. Its specificity, sensitivity, and versatility make it a valuable asset across various fields, from cancer biology to synthetic biology.

For more information, explore these trusted resources:

With the right tools and protocols, researchers can unlock new insights and advance scientific discovery in fluorescent protein research.